Opis stanowiska
Requirements:
The candidate should have PhD in biology (specialization in neurophysiology)
Conditions of employment: good knowledge of neurophysiology and chronobiology particularly in neuronal mechanism of circadian timing system, anatomy, physiology and electrophysiology of suprachiasmtic nucleus (SCN), intergeniculate leaflet (IGL) of the thalamus and dorsomedial hypothalamus (DMH); practical knowledge of electrophysiological recordings in vivo with using multi-unit recording technique and in vitro by using multi-electrode array (MEA) technique; immunohistochemistry / immunofluorescence techniques, laboratory experience in work with laboratory animals (rats), spoken and written Polish and English.
Description of tasks:
Post-doc will participate in the project: “Feeding time. Multidimensional studies on the impact of satiety signals on circadian timing system in the development of obesity.”
funded by the National Science Center (OPUS13) for 2018-2021.
The main aim of the proposed project is to investigate the impact of glucagon family peptides on the neuronal structures putatively involved in food intake and circadian rhythmicity, in both physiological conditions and under high-fat diet. For more information, please visit https://www.ncn.gov.pl/konkursy/wyniki/2017-11-15-opus13-preludium13
Tasks:
1. Immunohistochemical studies on the IGL-SCN-DMH axis in physiology and under high-fat diet. Periprandial variation in GLP-1/-2/OMX, GLP-1R/GLP-2R/GCGR and Fos immunoreactivity in the chosen brain structures.
2. Pharmacological screening with multielectrode array in vitro to check for possible differences in the firing rate and pattern of neurons in the IGL-SCN-DMH axis in the brain slices obtained from rats fed standard vs. high-fat diet
3. Electrophysiological multi-unit extracellular studies in vivo to check Responsiveness of IGL-SCN-DMH axis to GLP-1/-2/OXM present in the blood stream
Specific tasks:
Performing of electrophysiological recordings in vitro whole-cell patch clamp, multi-electrode array on rat brain slices and in vivo extracellular multi-unit recordings on urethane-anaesthetised rats. Immunohistochemical stainings with the use of fluorochrome-conjugated antibodies on the rat brain slices, scanned with confocal microscope.
Applications can be send to the following address: Project Investigator: Prof. dr hab. Marian H. Lewandowski, Department of Neurophysiology and Chronobiology, Institute of Zoology and Biomedical Research, Jagiellonian University. Gronostajowa 9, 30-387 Kraków or in electronic form to the e-mail address: marian.lewandowski@uj.edu.pl.
If you have any questions, please email to: marina.lewandowski@uj.edu.pl